《植物生理学报》 2014, 50(8): 1126-1134

影响农杆菌介导的杨树遗传转化技术的因素

陈仲^1,*, 廖维华^1,*, 王静澄², 高凯¹, 孙吉¹, 安新民^1,**

¹北京林业大学林木育种国家工程实验室, 林木花卉遗传育种教育部重点实验室, 北京100083; ²环境保护部环境规划院, 北京100012

通信作者：陈仲；E-mail: xinminan@163.com；Tel: 010-62336248

摘　要：

杨属树种是农杆菌的天然寄主之一, 但木本植物一般再生能力较差, 如何提高杨树的遗传转化频率就成为目前研究者最为关心的问题。本文结合农杆菌介导杨树遗传转化研究的最新进展和我们的实际工作对农杆菌的遗传转化原理、转移方法、以及影响农杆菌转化的因素进行了全新而深入的论述, 并针对如何提高木本植物的转化频率, 提出了一些改良的措施和方法。

关键词：农杆菌; 遗传转化; 杨树; 选择标记; 受体

收稿：2014-06-19   修定：2014-07-14

资助：国家“863”项目(2013AA102703和2011AA100201)和国家自然科学基金(31170631和J1103516)。

Factors Influencing Frequency of Agrobacterium-Mediated Populus Transformation

CHEN Zhong^1,*, LIAO Wei-Hua^1,*, WANG Jing-Cheng², GAO Kai¹, SUN Ji¹, AN Xin-Min^1,**

¹National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of the Ministry of Education, Beijing Forestry University, Beijing 100083, China; ²Chinese Academy for Environmental Planning, Ministry of Environmental Protection, Beijing 100012, China

Corresponding author: CHEN Zhong; E-mail: xinminan@163.com; Tel: 010-62336248

Abstract:

Populus is one of natural host of Agrobacterium. Due to the poor regenerative capacity of woody plants, how to enhance transgenic frequency in poplar is becoming a crucial problem. This review introduced the principles and methods of Agrobacterium-mediated transformation and discussed the key factors affecting genetic transformation in Populus, based on the latest progress and our practical work. Finally, this review provided some improved methods and measures for the improvement of the transformation frequency of woody plants.

Key words: Agrobacterium tumefaciens; genetic transformation; Populus; selectable marker; acceptor